how to calibrate micropipette

How to Calibrate a Micropipette?

How to Calibrate a Micropipette?

 

🔹 How to Calibrate a Micropipette – Step by Step

 

1. Prepare the Setup
  • Take an analytical balance.

  • Use distilled water and a clean container.

  • Record the room temperature.

2. Test the Volume
  • Set the micropipette to the desired volume (example: 100 µL).

  • Pipette distilled water and dispense it into the container.

  • The weight should match the expected value (1 µL ≈ 1 mg of water).

3. Repeat the Process
  • Do this at least 10 times for accurate results.

  • Test the minimum, middle, and maximum volumes.

4. Adjust if Needed
  • If the readings are not accurate, use the calibration screw to adjust.

  • Recheck until results are correct.

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